smiFISH - single molecule inexpensive fluorescence in situ hybridisation

A set consisting of short, target-binding, unlabelled oligo probes (OligoPool) and a double-labelled secondary probe allows extremely sensitive and specific in situ RNA detection.



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Take advantage of state-of-the-art synthesis technology:
Oligonucleotides with all popular modifications at reasonable prices and with a reliable quality. Oligos for PCR, short, unmodified and quickly delivered are offered as well as high quality RNA oligos, probes for qPCR and FISH or reliable cloning oligos of (nearly) any length.
Talking about oligonucleotides:
talk to biomers.net!

In order to increase the affinity and stability of a duplex DNA, there is a range of different modified bases to improve hybridisation:

- Minor Groove Binder (MGB) and LNA
- C-5 Propynyl-Pyrimidines (pdC, pdU)
- Aminoethyl-phenoxazine-deoxycytidine (AP-dC, (G-clamp))
- 5-Methyl-deoxycytidine (5-Me-dC)
- 2-Amino-deoxyadenosine (2-Amino-dA)

The new real-time PCR application is characterised by

- a high sensitivity,
- tremendous specificity and a
- high degree of flexibility

Oligonucleotide-based biosensors (E-sensors) for electrochemical detection can be labelled with

- Methylene blue
- Ferrocene or
- Anthraquinone
- Viologen
- Atto MB2

Between 6:00 and 9:30 am you‘ll get a special discount of
5 % on all your orders!

Protocols, interesting literature and further information around the topic of oligonucleotides.

- Smart-Seq for transkriptome analysis (Picelli et al., 2014)
- Mediator Probe PCR (Faltin et al., 2012)
- MiL-FISH: Multi-labelled oligonucleotides (Schimak et al., 2015)