Page id: 182

Literature highlights

1. Mediator Probe PCR

Fluorescence signal-to-noise optimisation for real-time PCR using universal reporter oligonucleotides
Michael Lehnert, Elena Kipf, Franziska Schlenker, Nadine Borst, Roland Zengerle, Felix von Stetten
Analytical Methods (2018), DOI: 10.1039/c8ay00812d.

Mediator Probe PCR: A novel approach for detection of Real-Time PCR based on label-free primary probes and standardized secondary universal fluorogenic reporters
Bernd Faltin, Simon Wadle, Günther Roth, Roland Zengerle, Felix von Stetten
Clinical Chemistry, 58:11 (2012); 1546-1556.

Using the mediator probe PCR, the authors present a cost-efficient and sequence-specific alternative, that will help to detect amplification of real-time PCR using universal fluorogenic reporters. 

2. Smart-seq2 Protocol

- Full-length RNA-seq from single cellls using Smart-seq2
Simone Picelli, Omid R Faridani, Åsa K Björklund, Gösta Winberg, Sven Sagasser and Rickard Sandberg
Nature Protocols, Vol. 9, No.1 (2014); 171-181.

The authors describe a detailed protocol with improved sensitivity and accuracy for transcriptome analysis from single cells comprising the generation of full-length cDNA and sequencing libraries.
The oligonucleotides are available from  

3. MiL-FISH: Multi-labelled Oligonucleotide

MiL-FISH: Multi-Labelled oligonucleotides for fluorescence in situ hybridisation improve visualization of bacterial cells
Mario P. Schimak, Manuel Kleiner, Silke Wetzel, Manuel Liebeke, Nicole Dubilier, Bernhard M. Fuchs
Applied and Enviromental Microbiology, (2015); 10.1128/AEM.02776-15.

Using MiL-FISH (multi-labelled FISH) the authors present  a multi-faceted alternative to standard mono-labelled FISH that can be used for a wide range of biological and medical applications. It enables the simultaneous multi-labelling of up to seven microbial groups and ensures a precise localisation of individual microbial cells with improved signal and imaging quality.