Literature highlights
 

SARS-CoV-2 detection - RT-LAMP

SARS-CoV-2 Detection Using an Isothermal Amplification Reaction and a Rapid, Inexpensive Protocol for Sample Inactivation and Purification
Brian A Rabe and Constance Cepko
medRxiv preprint doi: https://doi.org/10.1101/2020.04.23.20076877

Handyfuge-LAMP: low-cost and electricity-free centrifugation for isothermal SARS-CoV-2 detection in saliva
Ethan Li, Adam Larson, Anesta Kothari, Manu Prakash
medRxiv preprint doi: https://doi.org/10.1101/2020.06.30.20143255

Rapid Detection of Novel Coronavirus (COVID-19) by Reverse Transcription-Loop-Mediated Isothermal Amplification
Laura E. Lamb, Sarah N. Bartolone, Elijah Ward, Michael B. Chancellor
medRxiv preprint doi: https://doi.org/10.1101/2020.02.19.20025155

SARS-CoV-2 detection - RT-RPA

Rapid detection of SARS-CoV-2 by low volume real-time single tube reverse transcription recombinase polymerase amplification using an exo probe with an internally linked quencher (exo-IQ)
Ole Behrmann, Iris Bachmann, Martin Spiegel, Marina Schramm, Ahmed Abd El Wahed, Gerhard Dobler, Gregory Dame, Frank T Hufert
Clinical Chemistry, hvaa116, https://doi.org/10.1093/clinchem/hvaa116

Sortase mediated coupling of Gly-Gly-Gly-modified oligonucleotides to proteins

Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing
Brofelth, M., Ekstrand, A.I., Gour, S. et al.
Commun Biol 3, 339 (2020), https://doi.org/10.1038/s42003-020-1068-0

L-DNA oligonucleotides for DNA-PAINT

Left-handed DNA-PAINT for improved superresolution imaging in the nucleus
H.J. Geertsema, G. Aimola, V. Fabricius, J.P. Fuerste, B.B. Kaufer, H. Ewers
doi: https://doi.org/10.1101/2020.03.28.010553

Mediator Probe PCR

- Fluorescence signal-to-noise optimisation for real-time PCR using universal reporter oligonucleotides
Michael Lehnert, Elena Kipf, Franziska Schlenker, Nadine Borst, Roland Zengerle, Felix von Stetten
Analytical Methods (2018), DOI: 10.1039/c8ay00812d.

- Mediator Probe PCR: A novel approach for detection of Real-Time PCR based on label-free primary probes and standardized secondary universal fluorogenic reporters
Bernd Faltin, Simon Wadle, Günther Roth, Roland Zengerle, Felix von Stetten
Clinical Chemistry, 58:11 (2012); 1546-1556.

Using the mediator probe PCR, the authors present a cost-efficient and sequence-specific alternative, that will help to detect amplification of real-time PCR using universal fluorogenic reporters. 

Smart-seq2 Protocol

- Full-length RNA-seq from single cells using Smart-seq2
Simone Picelli, Omid R Faridani, Åsa K Björklund, Gösta Winberg, Sven Sagasser and Rickard Sandberg
Nature Protocols, Vol. 9, No.1 (2014); 171-181.

The authors describe a detailed protocol with improved sensitivity and accuracy for transcriptome analysis from single cells comprising the generation of full-length cDNA and sequencing libraries.
The oligonucleotides are available from biomers.net.