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We are closed during the holidays from 23. Dec. 2024 until 01. Jan. 2025. We will be back at your service on the 02. Jan. 2025! The last shipment before the holidays will leave us in the afternoon of 20. Dec. 2024. Please make sure that the delivery can be accepted at your place. We wish you a Merry Christmas and a Happy New Year.

RNase substrate

Sensitive substrate for quick detection of RNases starting at 98€

[Read more]
pH Indicator on the oligo

Carboxy-SNARF as a
5'-/3'-modification on the oligo enables pH-dependent color change.
Contact us for further information.
[Read more]

Maleimide oligos

stable and covalent coupling with thiols
[Read more]

Triglycine/Sortase

Sortase-mediated coupling reaction via triglycine-modified oligos

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XXL DNA

DNA oligo amounts from 10 to 500 mg, HPLC purification incl. [Read more]

New: Reactive modifiers

Reactive dyes and modifiers for postynthetic labeling of oligonucleotides: azide, alkyne, succinimidyl ester modifiers.
[Read more]

Pseudouridine

Pseudouridine and N1-Methyl-Pseudouridine modified oligonucleotides.
Please contact us!
[Read more]

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PCR-Blocker
 

PCR-Blocker

Blocker-oligos: PCR-Blocker and modifications for blocking the 3'-terminus of an oligonucleotide


Specific blocking of individual components in a reaction mixture can be a valuable tool for complex reaction processes.
These targeted blockages are used in PCR reactions or to prevent concatemer formation . As so-called PCR blocker oligonucleotides with a defined sequence they can inhibit the polymerase during elongation without participating as a primer itself.
More common is the use of blocking modifications in qPCR probes. As result of the modification the oligonucleotide probes lack the hydroxyl group at the 3'-end required for the extension in PCR.
Depending on different applications there are several possibilities for functionalized oligonucleotides . Below you will find a short overview with available modifications. 


3'-Spacer C3



Due to its small size a simple carbon chain does not influence other processes like hybridisation.
A good example of the preferred use of a C3 carbon spacer to block the 
3'-end of an oligonucleotide can be seen here.
 

 3´-Spacer C3


3'-Phosphat



Oligonucleotides do not bear terminal phosphate groups per se. But certainly terminal phosphate groups can be attached on request. Due to the requirement of a 3'-terminal hydroxyl group, many reactions can be blocked  by the addition of a phosphate group at the 3'-terminus of an oligonucleotide. 
 

 3´-Phosphate


3'-ddC



Since the establishment of Sanger sequencing, dideoxy nucleotides are widely used to stop polymerisation reactions. Currently only dideoxy cytidine (ddC) can be offered for oligonucleotide synthesis; for the other nucleotides are not available yet.
 

 dideoxy nucleotide 3´-ddC


3'-Inverted End



This modification consists of an inversely coupled nucleotide, generating an oligo with 5´-termini at both ends. 
Compared to the previous modifications, the addition of an entire nucleotide as blocking unit is more bulky. But like the phosphate group, it is a natural structure element, so that side-reactions during in vivo applications are not to be expected.
 

 inverted end


For further information and more details as well as pricing we are at your disposal at all times.
Tel  +49 731 70 396 0 | info@biomers.net