BMN Dyes and Quenchers
BMN Dyes– Dyes from biomers.net
BMN-488, BMN-505, BMN-536, BMN-562
In addition to the wide range of known fluorescent dyes, biomers.net also offers its own excellent alternatives.
The so-called BMN dyes can be attached to an oligonucleotide at the 5´- or 3´-end.
| Dye | ABS [nm] | EM [nm] | Alternative to |
|---|---|---|---|
| BMN-488 | 495 | 519 | Atto 488, Alexa Fluor 488® (identical structure) |
| BMN-505 | 507 | 524 | Fam, Fluorescein, FITC |
| BMN-536 | 536 | 553 | Carboxyrhodamin 6G, Atto Rho6G |
| BMN-562 | 562 | 578 | Rhodamin B |
| BMN-5 * | 645 | 671 | Cyanine 5 |
| BMN-6 * | 681 | 704 | Cyanine 5.5 |
* Due to the changed patent situation of the cyanines, we recommend the use of the analog cyanine dyes Cyanine 5 (analog BMN-5) and Cyanine 5.5 (analog BMN-6).
BMN Quencher – Quenchers from biomers.net
BMN-Q460, BMN-Q535, BMN-Q1, BMN-Q2, BMN-Q620
In addition to this wide range of available quenchers, biomers.net also offers a "proprietary" quencher set.
The BMN quenchers of biomers.net are excellent alternatives to the traditional Dark Quenchers due to their optimal matching quenching range.
| Quencher | ABS [nm] | Quenching Range [nm] | Alternative to |
|---|---|---|---|
| BMN-Q460 | 453 | 400-530 | Dabcyl, DDQ I |
| BMN-Q535 | 535 | 480-580 | BHQ-1, TQ2, DDQ I, Iowa Black FQ, Eclipse-Quencher, Dabcyl |
| BMN-Q1 | 535 | 480-580 | BHQ-1, TQ2, DDQ I, Iowa Black FQ, Eclipse-Quencher, Dabcyl |
| BMN-Q2 | 580 | 500-650 | BHQ-2, TQ3 |
| BMN-Q590 | 590 | 550-650 | will be discontinued |
| BMN-Q620 | 620 | 480-720 | BHQ-2, TQ3, BBQ-650, DDQ II, Iowa Black RQ |
| BMN-Q651 | 650 | 550-720 | BBQ-650, DDQ II |
Figure1: dT20 oligo with 3´-quencher, meaurement of absorption spectra in buffer (pH 7.0)
BMN-Q460
With an absorption maximum of 460 nm, our Dark Quencher BMN-Q460 is particularly suitable for the short-wavelength region and represents an excellent alternative for the quenchers Dabcyl and DDQ I.
In addition to use in Molecular Beacons, in which it inhibits fluorescent signals by contact-quenching, BMN-Q460 can be used in various molecular biological applications. As 3´-quencher in Dual-Labelled probes (5´-nuclease assay), BMN-Q460 can be used in the range of 400-530 nm, so it is ideally suited for the fluorophores Atto 390, Atto 425 and Atto 465.
biomers.net offers the quencher BMN-Q460 as 5´-, 3´- or internal modification.
BMN-Q535
BMN-Q535 is the second Dark Quencher of biomers.net and has an absorption maximum at about 535 nm and a quenching range from about 480 nm to 580 nm. This is the same range as BHQ-1, TQ2, DDQ I, Eclipse-Quencher or Iowa Black FQ, so BMN-Q535 may be an excellent alternative for this quenching.
BMN-Q535 is used as 3'-modification for standard Dual-Labelled Probes and Molecular Beacons.
We offer this quencher as 5 'or 3 modification. In addition, BMN-Q535 can be used as internal modification in Double Quenched probes with increased sensitivity.
| Dual-Labelled Probe | Double Quenched Probe | Molecular Beacon Probe |
|---|---|---|
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BMN-Q1 and BMN-Q2
BMN-Q1 and BMN-Q2 are direct alternatives with identical specifications to BHQ-1 and BHQ-2.
BMN-Q620
Similar to the quenchers BMN-Q460 and BMN-Q535, biomers.net also offers the dark quencher BMN-Q620 as
5´, 3´ and internal modification. Thus, BMN-Q620 can be used ideally in Double Quenched Probes for the longer wavelength region. With an absorption maximum of 620 nm and a quenching range of 480 nm to 720 nm, it has a relatively wide range and can therefore optimally quench fluorescence dyes also in the long-wave range, e.g. Cyanine 5 or Cyanine 5.5.
BMN-Q651
With an absorption maximum at 650 nm and a quenching range of 550-720 nm, the biomers.net quencher BMN-Q651 optimally covers the longer wavelength range. BMN-Q651 can be used as an alternative to BBQ-650 or DDQ II.
