DoubleQuenched Probes

Standard 5'-nuclease assay qPCR probes have a fluorophor at the 5'-end and an appropriate quencher at the 3'-end of the oligonucleotide. Besides quenching the emitted fluorescence of the 5'-dye, the quencher molecule also prevents elongation of the probe oligonucleotide during PCR.

For longer probes with increasing distance between fluorophor and quencher one may observe an increase of unwanted background. In these cases incorporation of an additional BMN quencher (e.g. BMN-Q460, BMN-Q535 or BMN-Q620) into the oligonucleotide sequence near the 5'-fluorophor highly improves the qPCR sensitivity and lowers the background.

DoubleQuenched Probes consist of a 5'-fluorophor, a 3'-BMN quencher and an additional internal BMN quencher which is in a distance of 8 - 10 bases from the fluorophor.

Dual-Labelled Probe	DoubleQuenched Probe
5'-Fam, 3'-BMN-Q535	5'-Fam, int. BMN-Q535, 3'-BMN-Q535

Standard Dual Labelled Probes as well as DoubleQuenched Probes with BMN-Q535 are available for online ordering; for DoubleQuenched Probes please have a look at the ordering information.

For any further information or help, please contact the biomers.net customer service.