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Unmodified DNA oligonucleotides
 

Standard scale for oligonucleotides

Standard oligonucleotides up to 140 bases

 

Standard oligonucleotides are provided by biomers.net in four different scales. Here please find an overview of the offered length, yields, and purifications:

Synthesis scale1 XS
0,02 µmol
S
0,05 µmol
M
0,2 µmol
L
1,0 µmol
     Length (bases) 12-60 12-140 12-140 6-140
     Wobbles - yes yes yes
     Purification2        
     Cartridge yes yes - -
     HPLC - yes yes yes
     PAGE - - yes yes
     Yield3        
     Cartridge/HPLC > 2 OD > 3 OD > 10 OD > 30 OD
     PAGE - - > 1 OD > 5 OD
     Quality control4        
     Maldi yes yes yes yes


1. The synthesis scale defines the amount of starting material, it is not the yield of the finalised oligo. Due to the many steps of synthesis, processing and purification the final yield is significantly lower.

2. Oligos are delivery exclusively with purification. At least a cartridge purification is applied. We do not offer unpurified oligo nor only desalted oligos. By default all oligonucleotides with more than 60 bases in standard scales S - L are purified by HPLC (optional PAGE may be chosen).

3. The yields provided at "guaranteed delivery amount" are valid for a mixed 20mer DNA-sequence. For significantly shorter or longer oligos as well as for homopolymers this yield may differ. According to our experience, the actual delivery amounts exceed the guaranteed yields significantly, certainly we deliver the total yield.

4. Routinely, each oligo up to a length of 50 bases will be checked for quality by Maldi mass spectrometry.

 

2 - 5mer oligos

2 - 5mer oligos

 

The actual synthesis process is identical for almost all lengths of oligonucleotides. Due to the small molecular size, very short oligos, however, differ in terms of the isolation and purification. We offer specific synthesis scales for di, tri, tetra and pentamer oligonucleotides including the corresponding adapted purification:

Synthesis scale DL2 DL3 DL4 DL5
     Length (bases) 2 3 4 5
     HPLC-purification yes yes yes yes
     Maldi-quality control yes yes yes yes
     Yield (µmol) approx. 0,2 0,2 0,2 0,2
 

Mid Scale DNA

Mid Scale DNA

 

Mid Scale DNA synthesis (XXL)
Oligonucleotide amounts from 10 mg to 500 mg.

Do you need more? No problem. We offer for our XXL scales oligonucleotide amounts from 10 mg to 500 mg.
All scales are valid for DNA oligonucleotides with a length of 12-40 bases without wobbles.

Synthese scale  XXL10MG XXL25MG XXL50MG XXL100MG XXL250MG XXL500MG
     Yield (mg) > 10 mg > 25 mg > 50 mg > 100 mg > 250 mg > 500 mg
     Yield (OD), ca. 400 OD 850 OD 1700 OD 3400 OD 8500 OD 17000 OD
     Yield (µmol) 20mer, ca. 1,8 µmol 3,5 µmol 7,5 µmol 15 µmol 38 µmol 77 µmol


In addition, we offer a wide range of modifications available for all our XXL DNA scales. 
For further information please contact our customer support service at any time. 
Tel +49 731 70 396 0   ǀ  info@biomers.net

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