Unmodified DNA oligonucleotides
Standard scale for oligonucleotides
Standard oligonucleotides up to 140 bases
Standard oligonucleotides are provided by biomers.net in four different scales. Here please find an overview of the offered length, yields, and purifications:
| Synthesis scale1 | XS 0,02 µmol |
S 0,05 µmol |
M 0,2 µmol |
L 1,0 µmol |
|---|---|---|---|---|
| Length (bases) | 12-60 | 12-140 | 12-140 | 6-140 |
| Wobbles | - | yes | yes | yes |
| Purification2 | ||||
| Cartridge | yes | yes | - | - |
| HPLC | - | yes | yes | yes |
| PAGE | - | - | yes | yes |
| Yield3 | ||||
| Cartridge/HPLC | > 2 OD | > 3 OD | > 10 OD | > 30 OD |
| PAGE | - | - | > 1 OD | > 5 OD |
| Quality control4 | ||||
| Maldi | yes | yes | yes | yes |
1. The synthesis scale defines the amount of starting material, it is not the yield of the finalised oligo. Due to the many steps of synthesis, processing and purification the final yield is significantly lower.
2. Oligos are delivery exclusively with purification. At least a cartridge purification is applied. We do not offer unpurified oligo nor only desalted oligos. By default all oligonucleotides with more than 60 bases in standard scales S - L are purified by HPLC (optional PAGE may be chosen).
3. The yields provided at "guaranteed delivery amount" are valid for a mixed 20mer DNA-sequence. For significantly shorter or longer oligos as well as for homopolymers this yield may differ. According to our experience, the actual delivery amounts exceed the guaranteed yields significantly, certainly we deliver the total yield.
4. Routinely, each oligo up to a length of 50 bases will be checked for quality by Maldi mass spectrometry.
2 - 5mer oligos
2 - 5mer oligos
The actual synthesis process is identical for almost all lengths of oligonucleotides. Due to the small molecular size, very short oligos, however, differ in terms of the isolation and purification. We offer specific synthesis scales for di, tri, tetra and pentamer oligonucleotides including the corresponding adapted purification:
| Synthesis scale | DL2 | DL3 | DL4 | DL5 |
|---|---|---|---|---|
| Length (bases) | 2 | 3 | 4 | 5 |
| HPLC-purification | yes | yes | yes | yes |
| Maldi-quality control | yes | yes | yes | yes |
| Yield (µmol) approx. | 0,2 | 0,2 | 0,2 | 0,2 |
Mid Scale DNA
Mid Scale DNA
Mid Scale DNA synthesis (XXL)
Oligonucleotide amounts from 10 mg to 500 mg.
Do you need more? No problem. We offer for our XXL scales oligonucleotide amounts from 10 mg to 500 mg.
All scales are valid for DNA oligonucleotides with a length of 12-40 bases without wobbles.
| Synthese scale | XXL10MG | XXL25MG | XXL50MG | XXL100MG | XXL250MG | XXL500MG |
|---|---|---|---|---|---|---|
| Yield (mg) | > 10 mg | > 25 mg | > 50 mg | > 100 mg | > 250 mg | > 500 mg |
| Yield (OD), ca. | 400 OD | 850 OD | 1700 OD | 3400 OD | 8500 OD | 17000 OD |
| Yield (µmol) 20mer, ca. | 1,8 µmol | 3,5 µmol | 7,5 µmol | 15 µmol | 38 µmol | 77 µmol |
In addition, we offer a wide range of modifications available for all our XXL DNA scales.
For further information please contact our customer support service at any time.
Tel +49 731 70 396 0 ǀ info@biomers.net
VAT and shipping costs may apply.
